CREST
ichikiCRESTの資料
進化実験全体の流れ Overview of On-chip Directed Evolution Procedure |
[140224JP] : 140224 Shusuke SATO
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GFP進化実験全体の流れ Procedure Overview : Directed Evolution of GFP |
[131027JP] : 131027 Shingo UENO
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BGL進化実験全体の流れ Procedure Overview : Directed Evolution of BGL |
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進化対象タンパク質の遺伝子/アミノ酸情報 Gene/AminoAcids sequence of Target Proteins |
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変異体DNAライブラリー作製法 Preparation of Mutant Library |
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変異体DNAライブラリーのシーケンシング DNA sequencing of Mutant Library |
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調製済み変異体DNAライブラリー情報 Information of Prepared Mutant Library |
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１分子エマルションPCR Single Molecule Emulsion PCR |
プロトコル
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クリーンルーム使用ルール Clean Room Rules |
日本語 [1304xxJP] : 1304xx Aiko ONO [140328JP] : 140328 Hiromi KURAMOCHI English [1304xxEN] : 1304xx Aiko ONO [140328EN] : 140328 Hiromi KURAMOCHI |
SPM洗浄プロトコル・試薬廃棄方法 SPM Cleaneing and Treatment of Waste Liquid |
[1304xxJP] : 1304xx Aiko ONO |
フォトマスク作製法 Preparation of Photomask |
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各種フォトマスク図面 Photomask Drawings |
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SU-8モールド作製法 Preparation of SU-8 Mold |
English [1307xxEN] : 1307xx Subhashini 日本語 [1309xxJP] : 1309xx Naoko TOMINAGA [140502JP] : 140502 Shingo UENO correction |
PDMSリアクター作製法 Preparation of PDMS Reactor |
[130412JP] : 130412 Aiko ONO [1309xxJP] : 1309xx Naoko TOMINAGA |
ガラスリアクター作製法 Preparation of Glass Reactor |
00 石英ガラス発注 (仙台石英) [150331JP] :150331 Akifumi YOSHIHARA 01 フォトマスク＆ウェットエッチング [140306JP] :140306 Tatsunori HIRAI 02 ドライエッチング [140306JP] :140306 Tatsunori HIRAI |
磁気ビーズ分配 Magnetic Beads Arraying |
[140306JP] :140306 Tatsunori HIRAI
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ガラス製マイクロアレイ中でのBGL合成・固定化 Preparation of BGL-immobilized Glass Reactor |
[140306JP] :140306 Tatsunori HIRAI |
送液デバイス作製法 Preparation of Fluidic Device for Cell-Free Synthesis |
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送液デバイス図面 Fluidic Device Drawing |
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送液デバイス アルミアダプター図面 Drawing of Aluminum Adaptor of the Fluidic Device (株式会社 庄内クリエート工業(蒲田営業所)に外注） |
送液デバイスアダプター図面
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ガラスヒーター図面 Glass Heater Drawing (株式会社ブラストに外注) |
[140314JP PDF],
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送液デバイス・無細胞転写反応 Operation of Fluidic Deivice & Cell-Free Synthesis |
[131111JP Beta] : 131014 Shingo UENO (参考ファイル) |
TDIスキャンマニュアル TDI Scanning |
[131014JP] : 131014 Shingo UENO [131026JP] : 131026 Shingo UENO [PDF] : 浜ホト簡易マニュアル |
アレイ解析ソフト Fluorescence Array Spots Analyzing Software |
[131001] : 131001 Keisaku HAMADA (Nikon Corp.), マニュアル付属 [131029] : 131029 Keisaku HAMADA (Nikon Corp.) |
アレイ解析法 Analysis of Fluorescence Array Spots |
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ガラスキャピラリー作製 Preparation of Glass capillary "Micropipette puller P-2000 (Sutter Instrument)" |
[1311111JP] : 131111 Shingo UENO |
磁気ビーズ回収方法 Collection of Magnetic Beads from Microwells |
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回収磁気ビーズからのDNA増幅 Amplification of DNA on Collected Beads |
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APTES修飾ガラス基板作製法 Preparation of APTES Modified Glass Substrate |
[121018JP] : 121018 Aiko ONO [130826 Modified by S.UENO] |
APTES基板評価プロトコル Evaluation of APTES Modification Quality |
[1304xxJP] : 1304xx Aiko ONO [130702JP] : 130702 Modified by Shingo UENO |
APTES基板へのDNA固定化 DNA immobilization onto APTES Modified Substrate |
[120508JP] : 120508 Aiko ONO [130409JP] : 130409 Aiko ONO |
mRNAの調製 Preparation of mRNA |
[130412JP] : 130412 Aiko ONO |
mRNAのマイクロインタリオプリンティング Microintaglio Printing of mRNA |
金修飾基板Ver.APTES修飾基板Ver. [130409JP] : 130409 Aiko ONO |
Ni-NTA修飾ガラス基板作製法 Preparation of Ni-NTA modified Glass Substrate |
[140306JP] : 140306 Tatsunori HIRAI [150331JP] : 150331 Akifumi YOSHIHARA |
タンパク質のマイクロインタリオプリンティング Microintaglio Printing of Protein |
[140306JP] : 140306 Tatsunori HIRAI |
基板上固定化DNAの光回収 Photo-assisted recovery of DNA from Substrate |
[130409JP] : 130409 Aiko ONO (Nitrobenzyl, APTES/Gold, NikonC1) |
Nikon C1 操作方法 Operation of Nikon-C1 |
[1304xxJP] 1304xx Aiko ONO |
DUV照射用光源装置 操作方法 Operation of Deep UV Irradiator |
[1309xxJP] 1309xx Nikon Engineering |
プラスミド情報 Plasmid information
p001 |
Data Sheet p001.xlsx : 120914 Shingo UENO p001-rev1.docx : 150129 Shingo UENO Sequence p001.fas : 150129 Shingo UENO Plasmid Map p001.pmd : 1501xx Shingo UENO Vector information (pGEM-T Easy) Sequence pGEMTeasy.rtf, Plasmid Map pGEMTeasy.gif |
p002 |
Data Sheet p002.xlsx : 121204 Shingo UENO p002-rev1.docx : 150129 Shingo UENO Sequence p002.fas : 150129 Shingo UENO Plasmid Map p002.pmd : 1501xx Shingo UENO Vector information (pET-28) Sequence pET28a+.txt, Plasmid Map pET28.pdf |
p003 |
Data Sheet p003.docx : 140717 Shingo UENO Sequence p003.fas : 150129 Shingo UENO Plasmid Map p003.pmd : 150129 Shingo UENO Vector information (pTAKN-2) pTAKN2.pdf |
p004 |
Data Sheet p004.docx : 140717 Shingo UENO Sequence p004.fas : 150129 Shingo UENO Plasmid Map p004.pmd : 150129 Shingo UENO Vector information (pTAKN-2) pTAKN2.pdf |
p005 |
Data Sheet p005.docx : 150129 Shingo UENO Sequence p005.fas : 150129 Shingo UENO Plasmid Map p005.pmd : 150129 Shingo UENO Vector information (pGEM-T Easy) Sequence pGEMTeasy.rtf, Plasmid Map pGEMTeasy.gif |
p006 |
Data Sheet p006.docx : 150129 Shingo UENO Sequence p006.fas : 150129 Shingo UENO Plasmid Map p006.pmd : 150129 Shingo UENO Vector information (pTAKN-2) pTAKN2.pdf |
p007 |
Data Sheet p007.docx : 150129 Shingo UENO Sequence p007.fas : 150129 Shingo UENO Plasmid Map p007.pmd : 150129 Shingo UENO Vector information (pTAKN-2) pTAKN2.pdf |
p008 |
Data Sheet p008.docx : 150129 Shingo UENO Sequence p008.fas : 150129 Shingo UENO Plasmid Map p008.pmd : 150129 Shingo UENO Vector information (pTAKN-2) pTAKN2.pdf |
p009 |
Data Sheet p009.docx : 150731 Shingo UENO Sequence p009.fas : 150731 Shingo UENO Plasmid Map p009.pmd : 150731 Shingo UENO Vector information (pTAKN-2) pTAKN2.pdf |
Last Updated: 2015-4-1 by S. UENO
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